Enzyme-free, signal-amplified nucleic acid circuits for biosensing and bioimaging analysis.

Jiyun Chen, Lijuan Tang, Xia Chu, Jianhui Jiang,

The Analyst, July 26, 2017

Enzyme-free, signal-amplified nucleic acid circuits utilize programmed assembly reactions between nucleic acid substrates to transduce a chemical input into an amplified detection signal. These circuits have shown great potential for developing biosensors for high-sensitivity and high-selectivity detection of varying targets including nucleic acids, small molecules and proteins in vitro and for high-contrast in situ visualization and imaging of these targets in tissues and living cells. We review the background of the enzyme-free, signal-amplified nucleic acid circuits, including their mechanism, significance, types and development. We also review current applications of these circuits for biosensors and bioimaging.

Pubmed Link: 28744530

DOI: 10.1039/c7an00967d