An informatic framework for decoding protein complexes by top-down mass spectrometry.

Owen S Skinner, Pierre C Havugimana, Nicole A Haverland, Luca Fornelli, Bryan P Early, Joseph B Greer, Ryan T Fellers, Kenneth R Durbin, Luis H F Do Vale, Rafael D Melani, Henrique S Seckler, Micah T Nelp, Mikhail E Belov, Stevan R Horning, Alexander A Makarov, Richard D LeDuc, Vahe Bandarian, Philip D Compton, Neil L Kelleher,

Nature methods, January 18, 2016

Efforts to map the human protein interactome have resulted in information about thousands of multi-protein assemblies housed in public repositories, but the molecular characterization and stoichiometry of their protein subunits remains largely unknown. Here, we report a computational search strategy that supports hierarchical top-down analysis for precise identification and scoring of multi-proteoform complexes by native mass spectrometry.

Pubmed Link: 26780093

DOI: 10.1038/nmeth.3731