Induction of Tolerance against the Arthritogenic Antigen with Type-II Collagen Peptide-Linked Soluble MHC Class II Molecules.

Yoon-Kyung Park, Sundo Jung, Se-Ho Park,

BMB reports, January 18, 2016

In murine collagen-induced arthritis (CIA), self-reactive T cells recognize peptide antigens derived from type-II collagen (CII). Activation of T cells is an important mediator of autoimmune diseases, and thus, T cells became a focal point of study to treat autoimmune diseases. In this study, we evaluated the efficacy of recombinant MHC class II molecules in the regulation of antigen-specific T cells by using a self peptide derived from CII (CII260-274; IAGFKGEQGPKGEPG) linked to mouse I-Aq in a murine CIA model. We found that recombinant I-Aq/CII260-274 molecules can be recognized by CII-specific T cells and can inhibit the same T cells in vitro. Furthermore, the development of CIA in mice was successfully prevented by the in vivo injection of recombinant I-Aq/CII260-274 molecules. Thus, treatment with recombinant soluble MHC class II molecules in complex with an immunodominant self-peptide offers a potential therapeutic for chronic inflammation in autoimmunity, such as rheumatoid arthritis.

Pubmed Link: 26779996