Method For Detecting Mutations In Single Cells Or Single Molecules

Document № Publication date Application №
20160046999 February 18, 2016 14/774543

The invention provides a high efficiency fluorescence in situ hybridization (FISH) method for detecting mutations on individual RNA transcripts, including both exonic and intronic RNA transcripts. In certain embodiments, the method is used to quantify allelic expression at the population and single cell level, and also to distinguish maternal chromosomes from paternal chromosomes in single cells.