Method For Detecting Mutations In Single Cells Or Single Molecules
|Document №||Publication date||Filed Date||Application №|
|20160046999||February 18, 2016||March 13, 2014||14/774543|
The invention provides a high efficiency fluorescence in situ hybridization (FISH) method for detecting mutations on individual RNA transcripts, including both exonic and intronic RNA transcripts. In certain embodiments, the method is used to quantify allelic expression at the population and single cell level, and also to distinguish maternal chromosomes from paternal chromosomes in single cells.